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Description:

Chicken Insulin-like Growth Factor-I (Chicken IGF-I) is a polypeptide comprising 70 amino acids, eight of which differ from the amino acid sequence of human IGF-I. Chicken IGF-I has been shown to stimulate growth in many cell types, including rat L6 myoblasts and chicken embryo fibroblasts, hepatocytes, adipocytes and myoblasts. GroPep’s Receptor Grade chicken IGF-I is a high quality research reagent particularly suited for use in avian cells and in vivo growth studies or for other general research purposes.

References:

Upton F.Z. et al. (1992) J. Mol. Endocrinol. 9, 83-92
McMurtry J.P. et al. (1997) Dom. Animal Endocrinol. 14, 199-229

Source:

Produced recombinantly in E.coli

Purity:

> 95 % (by HPLC and N-terminal sequence analysis)

Molecular Weight:

7738 daltons – confirmed by Mass Spectrometry

N-terminal sequence analysis:

5 residues > 95 % single sequence

Biological Activity:

Type 1 IGF receptor binding assay: ED₅₀ < 10 ng/ml
Stimulation of protein synthesis in rat L6 myoblasts:
ED₅₀ < 30 ng/ml

Endotoxin:

< 0.1 EU/µg

State and Appearance:

Lyophilized white powder
Dried from 0.1 M acetic acid and stored under dry nitrogen at a slight vacuum (-25 kPa)

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized)

Reconstitution:

#1000: Handling of Novozymes GroPep IGF-I, IGF-II and IGF Analogs

Detection:

#3001: Procedure for the Iodination of IGFs

#3002: Determination of IGF-I or IGF-II in a Range of Species by Radioimmunoassay (RIA)

#3003: Procedure for Western Ligand Blotting using iodinated IGF-I or IGF-II

Related Products:

IGF-I, anti-human

Any combination of 3 different IGF-Is and/or IGF-I analogs (all Receptor Grade)

Chicken IGF-I pre-RIA Pack

Chicken IGF-I & IGF-II Pack

Chicken IGF-I Pack

Product Performance:
      Protein Synthesis:




      Radioreceptor Assay:




      IGF Specificity Curves:




      Chicken IGF-I binding of 125I-Labelled IGF-I: