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Description:
Barramundi (Lates calcarifer) Insulin-like Growth Factor-I (Barramundi IGF-I) is a 66 amino acid polypeptide. While IGF-I is known to stimulate growth and differentiation in many cell types and to mediate many of the anabolic properties of growth hormone, the status of the IGF-I / growth hormone axis is currently under investigation in fish.
References:
Kinholt A. (1996) Barramundi (Lates calcarifer) IGF-I:
Characterization of cDNA, genosequences and regulation of mRNA expression.
PhD Thesis, Queensland University of Technology, Brisbane, Australia.
Degger, B. et al. (2000) Gen. Comp. Endocrinol. 117, 395-403
Source:
Produced recombinantly in E.coli
Purity:
> 95 % (by HPLC and N-terminal sequence analysis)
Molecular Weight:
7309 daltons – confirmed by Mass Spectrometry
N-terminal sequence analysis:
5 residues > 95 % single sequence
Biological Activity:
Stimulation of protein synthesis in rat L6 myoblasts:
ED50 < 30 ng/ml
Endotoxin:
< 0.1 EU/µg
State and Appearance:
Lyophilized white powder
Dried from 0.1 M acetic acid and stored under nitrogen at a slight vacuum
Storage/Stability:
At least 2 years at 2 - 4°C (lyophilized)
Reconstitution:
#1000: Handling of Novozymes GroPep IGF-I, IGF-II and IGF Analogs
Detection:
#3001: Procedure for the Iodination of IGFs
#3002: Determination of IGF-I or IGF-II in a Range of Species by Radioimmunoassay (RIA)
#3003: Procedure for Western Ligand Blotting using iodinated IGF-I or IGF-II
#4100: Determining fish IGF-I levels using the Novozymes GroPep Fish IGF-I RIA Kit
#4110: Immunohistochemical (IHC) Detection of IGF-I in paraffin sections of Barramundi (fish) kidney by Immunoperoxidase staining
Related Products:
IGF-I, anti-barramundi (fish)
IGF-I, antibody for Immunohistochemistry
Barramundi IGF-I Pack
IGF-I Immunohistochemistry Pack
Product Performance:
Protein Synthesis:
Protein Synthesis:
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