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Source:

Rabbit antiserum

Immunogen:

Epitope of human IGFBP-rP1

State and Appearance:

Lyophilized powder

Specificity:

As determined by EIA:
Antibody does not recognize IGFBPs 1,2,3,4,5,or 6

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized)
After reconstitution store at -20°C or -80°C
Avoid freeze-thaw cycles

Reconstitution:

Dissolve in 500 µl sterile water (a 1/5 dilution). Dilute in a buffer suitable for the desired application

Application and Titre:

Enzyme immunoassay 1/150,000
Half-maximal inhibition (ED₅₀) of radioligand binding is produced by < 10 ng hIGFBP-rP1/ 100 µl.
Western Blotting 1/4,000
Under reducing conditions.

Protocol:

#3110: Protocol for Western Immunoblotting using human IGFBP antisera

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