Biotech Reagents Ordering >> Product Families >> IGF Binding Proteins (IGFBP) >>

References:

Liu, S. et al. (1991) Biochem. Biophys. Res. Communs. 174 , 673-679
Schuller A.G. et al.(1993) Growth Regulation 3, 32-34

Source:

Purified IgG from rabbit antiserum

Immunogen:

Epitope of human IGFBP-1

State and Appearance:

Lyophilized powder

Specificity:

As determined by EIA:
Antibody does not recognize hIGFBPs 2,3,4,5 or 6

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized)
After reconstitution store at -20°C or -80°C
Avoid freeze-thaw cycles

Reconstitution:

Dissolve in 400 µl sterile water (a 1/20 dilution). Dilute in a buffer suitable for the desired application

Application and Titre:

Recognises IGFBP-1 in frozen and paraffin embedded human and rat tissue
Enzyme immunoassay 1/500 – 1/800
IHC fetal rat tissue 1/250

Protocol:

#3100: Immunohistochemical (IHC) Detection of IGFBPs In Paraffin Sections Of Rat Embryonic Tissues Using Extravidin-Cy3 Staining

#3102: Immunohistochemical (IHC) detection of human or rat IGFBPs and IGF-I, and rat IGF-II in formalin fixed, paraffin embedded human and rat tissue sections by horseradish peroxidase staining

Related Products:

Human IGFBP-1 (Native)

IGFBP-1 Immunohistochemistry Pack

Product Performance:
      IGFBP-1 in human liver metastasis of gastric carcinoma, intestinal type:




      IGFBP-1 in embryonic rat skin:




      IGFBP-1 in rat kidney:




      Negative Control: Non-specific Binding:

Antibody dilutions as per immune serum
Secondary: Biotinylated Anti-rabbit IgG
Detection: ExtrAvidin-Cy3 conjugate