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Description:

This Rat/Mouse IGF-I ELISA Kit has been jointly developed by GroPep Limited and ImmunoDiagnostic System Limited (IDS Ltd) for the quantitative determination of IGF-I levels in rat/mouse serum or plasma.

The Kit has been manufactured according to ISO9001:2000 / ISO13485:2003 Certified IDS Quality System.

IDS Limited website: www.idsltd.com

Insulin-like growth factor (IGF-I) is a naturally produced molecule of approximately 70 amino acids that stimulates growth and differentiation in many cell types. Measuring IGF-I is useful for investigating and monitoring a number of biological processes, including the actions of growth hormone, many of which are mediated by IGF-I.

Sufficient for 42 rat/mouse serum samples and Calibrators (in duplicate)
The method incorporates a sample pre-treatment to avoid interference from IGF binding proteins.

Specificity:

Suitable for measuring IGF-I in rat and mouse serum and plasma.

No interference from the following up to and including:
Rat IGFBP-4: 8000 ng/ml
Human IGF-II: 2000 ng/ml
Rat IGF-II: 2000 ng/ml
Bilirubin: 300 µg/ml
Lipid (triglyceride): 2000 mg/dl
Haemoglobin: 1470 mg/dl

Human IGF-I not detected up to 51885 ng/ml
(equivalent to < 0.01 % cross-reactivity)

Application and Titre:

Limit of detection: 82 ng/ml rat or mouse IGF-I

Accuracy and Reproducibility:
Reproducibility within run (CV): < 8 %
(n=20)
Reproducibility between runs (CV): < 13 %
(n=9)
For research use only. Not for use in diagnostic procedures.

Documentation:

Rat/Mouse IGF-I ELISA Kit Flyer

Rat/Mouse IGF-I ELISA Kit Support Document

Rat/Mouse IGF-I ELISA Kit Insert