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Source:

Antiserum (Rabbit)

Immunogen:

Recombinant rat IGF-I

State and Appearance:

Lyophilized powder

Specificity:

As determined by RIA, using PAT2:
100 % rat and mouse IGF-I
<0.05 % human IGF-I, chicken IGF-I
0 % rat IGF-II, human IGF-II

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized)
After reconstitution, aliquot and store at -20°C
Avoid freeze-thaw cycles

Reconstitution:

PAT2 (for RIA): Dissolve the vial contents in 250 µl RIA buffer (30 mM sodium phosphate pH 7.5 containing 10 mM EDTA, 0.2 g/l protamine sulphate, 0.5 ml Tween 20, 0.25 g/l sodium azide) to give an antiserum stock of 1/50.

Application and Titre:

Radioimmunoassay 1/60,000
At a dilution of 1/60,000, the antiserum binds 25% of fresh [¹²⁵I]rat IGF-I tracer.
Half-maximal inhibition (ED₅₀) of radioligand binding is produced by 0.2 ng of rat IGF-I.
For Western Immunoblotting use anti-rat IGF-I (PAT1). Dilution 1/1,000
For IHC, use IGF-I Antibody for immunohistochemistry. Dilution 1/50 - 1/200

Protocol:

#3002: Determination of IGF-I or IGF-II in a Range of Species by Radioimmunoassay (RIA)

Related Products:

Rat IGF-I

IGF-I, anti-rat (for Immunoblotting)

IGF-I, antibody for Immunohistochemistry

Rat IGF-I pre-RIA Pack

Rat IGF-I Pack

Product Performance:
      Cross-Reactivity: