Biotech Reagents Ordering >> Full Product List >>

Description:

Human Insulin-like Growth Factor Binding Protein-6 (IGFBP-6) is a 216 amino acid glycosylated protein that is one of the six “classical” IGF binding proteins that play an important role in stabilizing and regulating the activity of IGFs in vivo. Human IGFBP-6 binds IGF-II with a 20-70 fold higher affinity than IGF-I. The major glycosylated species in the product contains 216 amino acids, but a minor isoform with a 2 amino acid N-terminal extension is produced as a result of alternative processing by the CHO cells used for the rhIGFBP-6 production.

References:

Neumann G. M. et al. (1998) Biochemistry 37, 6572-6585
Zapf J. (1995) Eur. J. Endocrinol. 132, 645-654

Source:

Produced recombinantly in mammalian cells. Purified by affinity and other chromatographic steps.

Purity:

> 95% (by HPLC)

Molecular Weight:

28-30 kDa (glycosylated) Confirmed by SDS-PAGE

Biological Activity:

Single species detected by Western ligand blot using ¹²⁵I-IGF-II as probe

State and Appearance:

Lyophilized white powder dried from 10mM HCl and stored under dry nitrogen at a slight vacuum (-25 kPa)

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized)

Reconstitution:

#1100: Handling of GroPep Bioreagents IGF Binding Proteins 1-6

Detection:

#3100: Immunohistochemical (IHC) Detection of IGFBPs In Paraffin Sections Of Rat Embryonic Tissues Using Extravidin-Cy3 Staining

#3110: Protocol for Western Immunoblotting using human IGFBP antisera

Related Products:

IGFBP-6, anti-human

IGFBP-6, antibody for Immunohistochemistry

Human IGFBP-6 Pack

IGFBP-6 Immunohistochemistry Pack