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Description:

Human Insulin-like Growth Factor Binding Protein-5 (IGFBP-5) is a 252 amino acid protein that is one of the six “classical” IGF binding proteins that play an important role in stabilizing and regulating the activity of IGFs in vivo. Like hIGFBP-3 it forms a ternary complex with an acid-labile subunit (ALS). hIGFBP-5 preferentially binds IGF-II and it also binds with high affinity to extracellular matrix components which protect it from proteolysis. IGFBP-5 has a nuclear targeting sequence and, independent of the presence of IGF-I, fluorescently labeled IGFBP-5 is translocated to the nucleus of actively dividing cells. The significance of this observation is not yet understood.

References:

Kiefer M. et al. (1991) Biochem. Biophys. Res. Commun. 176, 219-225
Twigg S.M. et al. (1998) J. Biol. Chem. 273, 28791-28798

Source:

Produced recombinantly in mammalian cells. Purified by affinity and other chromatographic steps.

Purity:

> 95% (by HPLC and N-terminal sequencing)

Molecular Weight:

31 - 32 kDa

Biological Activity:

Detected by immunoblot using an antibody specific for human IGFBP-5.

State and Appearance:

Lyophilized white powder dried from 0.1 M acetic acid and stored under dry nitrogen at a slight vacuum (-25 kPa).

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized).

Reconstitution:

#1100: Handling of Novozymes GroPep IGF Binding Proteins 1-6

Detection:

#3100: Immunohistochemical (IHC) Detection of IGFBPs In Paraffin Sections Of Rat Embryonic Tissues Using Extravidin-Cy3 Staining

#3110: Protocol for Western Immunoblotting using human IGFBP antisera

Related Products:

IGFBP-5, anti-human

IGFBP-5, antibody for Immunohistochemistry

Human IGFBP-5 Pack

IGFBP-5 Immunohistochemistry Pack