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References:

Upton Z. et al. (1996) J. Endocrinol. 149, 379-397

Source:

Antiserum (Rabbit)

Immunogen:

An immunogenic analog of human IGF-I

State and Appearance:

Lyophilized powder

Specificity:

As determined by RIA:
100 % human, bovine, porcine IGF-I
100 % [Arg³]IGF-I
70 % chicken IGF-I
25 % rat IGF-I
20 % LONG^TMR³IGF-I
< 1 % human IGF-II

Storage/Stability:

At least 6 months at 2 - 4°C (lyophilized)
After reconstitution, aliquot the stock solution and store at -20°C
Avoid freeze-thaw cycles

Reconstitution:

Dissolve the vial contents in 250 µl RIA buffer (30 mM sodium phosphate pH 7.5 containing 10 mM EDTA, 0.2 g/l protamine sulphate, 0.5 ml Tween 20, 0.25 g/l sodium azide) to give an antiserum stock of 1/50.

Application and Titre:

Radioimmunoassay 1/80,000
At a dilution of 1/80,000 the antiserum binds 30 - 40 % of fresh [¹²⁵I]hIGF-I tracer.
Half-maximal inhibition (ED₅₀) of radioligand binding is produced by 250 - 300 pg of hIGF-I.
For IGF-I Immunohistochemistry use GroPep antibody
Product Code: PABCa

Protocol:

#3002: Determination of IGF-I or IGF-II in a Range of Species by Radioimmunoassay (RIA)

Related Products:

Human IGF-I

IGF-I, antibody for Immunohistochemistry