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Description:

Human Insulin-like Growth Factor Binding Protein-4 (IGFBP-4) is a 237 amino acid glycosylated protein that is one of the six “classical” IGF binding proteins that play an important role in stabilizing and regulating the activity of IGFs in vivo. Human IGFBP-4 is a 24-28 kDa glycoprotein that does not appear to associate with the cell surface or the extracellular matrix.

References:

Neumann G. M. et al. (1998) Biochemistry 37, 6572-6585
Zapf J. (1995) Eur. J. Endocrinol. 132, 645-654

Source:

Produced recombinantly in mammalian cells.
Purified by affinity chromatographic steps.

Purity:

>90% (by N-terminal sequencing)

Molecular Weight:

24-28 kDa (glycosylated) Confirmed by SDS-PAGE under reducing conditions.

Biological Activity:

Single species detected by Western ligand blot using ¹²⁵I-IGF-I as probe.

State and Appearance:

Lyophilized white powder dried from 0.1 M acetic acid and stored under dry nitrogen at a slight vacuum (-25 kPa).

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized).

Reconstitution:

#1100: Handling of GroPep Bioreagents IGF Binding Proteins 1-6

Detection:

#3100: Immunohistochemical (IHC) Detection of IGFBPs In Paraffin Sections Of Rat Embryonic Tissues Using Extravidin-Cy3 Staining

#3110: Protocol for Western Immunoblotting using human IGFBP antisera

Related Products:

IGFBP-4, anti-human

IGFBP-4, antibody for Immunohistochemistry

Human IGFBP-4 Pack

IGFBP-4 Immunohistochemistry Pack