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Description:

Human [Arg⁶]Insulin-like Growth Factor-II [Arg⁶]IGF-II) is an analog of human IGF-II, which has an Arg substitution in place of Glu at position 6 of the IGF-II amino acid sequence. It is therefore the IGF-II equivalent of [Arg³]IGF-I. [Arg⁶]IGF-II exhibits reduced affinity for IGF binding proteins (IGFBPs) compared with IGF-II and has a slightly greater affinity than Des(1-6)IGF-II for the Type 2 IGF receptor.

References:

Francis G. L. et al. (1993) Biochem. J., 293, 713-719

Source:

Produced recombinantly in E.coli

Purity:

> 95 % (by HPLC and N-terminal sequence analysis)

Molecular Weight:

7496 daltons – confirmed by Mass Spectrometry

N-terminal sequence analysis:

7 residues > 95 % single sequence

Biological Activity:

Type 2 IGF receptor binding assay: ED₅₀ < 100 ng/ml
IGF binding protein assay: ED₅₀ > 1000 ng/ml
Stimulation of protein synthesis in rat L6 myoblasts: ED₅₀ < 100 ng/ml

Endotoxin:

< 0.1 EU/µg

State and Appearance:

Lyophilized white powder.
Dried from 0.1 M acetic acid and stored under dry nitrogen at a slight vacuum (-25 kPa)

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized)

Article:

#1000: Handling of Novozymes GroPep IGF-I, IGF-II and IGF Analogs