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Description:

Rat Insulin-like Growth Factor-II (Rat IGF-II) is a polypeptide of 67 amino acids, four of which differ from the amino acid sequence of human IGF-II. Three of these occur in the C-domain – at position 32, 35 and 36; the other is at position 22 in the B-domain.

References:

Dull T.J. et al. (1986) Nature, 310, 777-781

Source:

Produced recombinantly in E.coli.

Purity:

> 95 % (by HPLC and N-terminal sequence analysis)

Molecular Weight:

7515 daltons – confirmed by Mass Spectrometry

N-terminal sequence analysis:

5 residues > 95 % single sequence

Biological Activity:

Type 2 IGF receptor binding assay: ED₅₀ < 5 ng/ml
Stimulation of protein synthesis in rat L6 myoblasts: ED₅₀ < 20 ng/ml

Endotoxin:

< 0.1 EU/ µg

State and Appearance:

Lyophilized white powder.
Dried from 0.1 M acetic acid and stored under dry nitrogen at a slight vacuum (-25 kPa)

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized).

Reconstitution:

#1000: Handling of Novozymes GroPep IGF-I, IGF-II and IGF Analogs

Protocols:

#3001: Procedure for the Iodination of IGFs

#3002: Determination of IGF-I or IGF-II in a Range of Species by Radioimmunoassay (RIA)

#3003: Procedure for Western Ligand Blotting using iodinated IGF-I or IGF-II

#3007: Procedure for Western Immunoblotting rat IGF-II

#3060: Immunohistochemical (IHC) Detection of IGF-II in paraffin embedded sections of fetal rat tissues

Related Products:

Rat IGF-II Pack

IGF-II, antibody for Immunohistochemistry

IGF-II, anti-human

Rat IGF-I & IGF-II Pack

Rat IGF-I