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Description:

Mono-biotinyl- and di-biotinyl- human Insulin-like Growth Factor-II (mono-biotinyl-human-IGF-II and di-biotinyl-human-IGF-II) are made by reacting hIGF-II with Sulfo-NHS-Biotin reagent. In mono-biotinyl-human-IGF-II, the a-amino group of the N-terminal alanine is biotinylated. In di-biotinyl-human-IGF-II both the a-amino group of the N-terminal alanine and the e-amino group of the lysine at position 65 are biotinylated.

References:

Fowlkes J. L. & Serra D. (1996) Endocrinol. 137, 5751-5754.

Source:

Following the reaction of hIGF-II with Sulfo-NHS-Biotin reagent , the mono- and di-biotinyl-human-IGF-II are separated from the other reaction products by HPLC. The mono-biotinyl-human-IGF-II and the di-biotinyl-human-IGF-II peaks are then further purified by HPLC to yield Receptor Grade products.

Purity:

> 95 % (by HPLC)

Molecular Weight:

Mono-biotinyl-human-IGF-II – 7696 daltons
Di-biotinyl-human-IGF-II – 7922 daltons
Homogeneity and mass of both confirmed by Mass Spectrometry.

N-terminal sequence analysis:

5 residues > 95 % single sequence

Biological Activity:

Western ligand blot to bovine IGFBP-2.

Endotoxin:

< 0.1 EU/µg

State and Appearance:

Lyophilized white powder.
Dried from 0.1 M acetonitrile/ 0.1% trifluoroacetic acid and stored under dry nitrogen at a slight vacuum (-25 kPa)

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized).

Detection:

By Western Ligand Blot

Reconstitution:

#1000: Handling of GroPep Bioreagents IGF-I, IGF-II and IGF Analogs

Protocol:

#3004: Procedure for Western Ligand Blotting using biotinylated IGF-I or IGF-II

Related Products:

Biotinyl human IGF-I