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Description:

GroPep scientists and engineers have produced Animal/Media Grade human IGF-II to provide a high quality reagent for use in studies on cellular and animal growth and for inclusion in cell culture medium.

References:

Humbel R.E. (1990) Eur. J. Biochem. 190, 445-462
Stewart C.E.H. & Rotwein P. (1996) Physiol. Rev. 76, 1005-1026

Source:

Produced recombinantly in E.coli.

Purity:

>90 % (by HPLC and N-terminal sequence analysis)

Molecular Weight:

7469 daltons – homogeneity confirmed by Mass Spectrometry

N-terminal sequence analysis:

5 residues > 95 % single sequence

Biological Activity:

Type 1 IGF receptor binding assay: ED₅₀ < 20 ng/ml
Type 2 IGF receptor binding assay: ED₅₀ < 70 ng/ml
IGF binding protein assay: ED₅₀ < 10 ng/ml
Stimulation of protein synthesis in rat L6 myoblasts: ED₅₀ < 150 ng/ml

Endotoxin:

< 0.1 EU/µg

State and Appearance:

Lyophilized white powder.
Dried from 0.1 M acetic acid and stored under nitrogen at a slight vacuum.

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized).

Reconstitution:

#1000: Handling of GroPep Bioreagents IGF-I, IGF-II and IGF Analogs

Detection:

#3002: Determination of IGF-I or IGF-II in a Range of Species by Radioimmunoassay (RIA)