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Description:
Human LONG™R³ Insulin-like Growth Factor-I (IGF-I) is an 83 amino acid analog of human IGF-I comprising the complete human IGF-I sequence with the substitution of an Arg for the Glu at position 3 (hence R³), and a 13 amino acid extension peptide at the N-terminus.
Recombinant LONG™R³IGF-I has been developed specifically for supplementation of mammalian cell culture to support the survival and proliferation of cells. It is engineered to have a higher biological potency than native IGF-I or IGF-II and has several advantages over recombinant insulin, potency being among them (1). Published research has shown that supplementation of cell cultures with LONG™R³IGF-I at a much lower concentration results in equivalent or better productivity than supplementation with standard concentrations of insulin. LONG™R³IGF-I is better able to stimulate the Type I IGF receptor and thus induce a higher level of activation of intracellular signaling molecules which are responsible for promoting cell survival by inhibition of apoptosis (1).
*SAFC Biosciences has exclusive marketing and distribution rights for LONG™R3IGF-I for use in industrial mammalian cell culture.
For information on using or ordering LONG™R³IGF-I for industrial cell culture, please click here or visit the SAFC Biosciences website: www.SAFCBiosciences.com
LONG is a trademark owned by GroPep Limited
LONGTMR3IGF-I is covered by the following patents assigned to GroPep:
US Patent 5,330,971 and 5,164,370; European Patent 429,586; Japanese Patent 2,682738, Australian Patent 633,099; Canadian Patent 2,033,176
References:
1. Yandell C., et al. (2004) An analogue of IGF-I. BioProcess International 2, 56-64
Source:
Produced recombinantly
Purity:
> 85 % (by HPLC)
Molecular Weight:
9110 +/- 2 daltons – confirmed by Mass Spectrometry
N-terminal sequence analysis:
18 residues > 95 % single sequence
Biological Activity:
Type 1 IGF receptor binding assay: ED50 > 15 ng/ml
IGF binding protein assay: ED50 > 200 ng/ml
Stimulation of protein synthesis in rat L6 myoblasts: ED50 < 10 ng/ml
Endotoxin:
< 0.1 EU/µg
State and Appearance:
Lyophilized white powder
Dried from 100 mM acetic acid and stored under nitrogen at a slight vacuum
Storage/Stability:
At least 2 years at 2 - 4°C (lyophilized)
Reconstitution:
100 mM acetic acid at a concentration of > 1 mg/ml
Reconstitution:
#1200: Handling of Novozymes GroPep LONG®R³IGF-I for use in Cell Culture
Detection:
#3030 Procedure for Human LONG®R³IGF-I Double monoclonal ELISA (BK / MAN1 / MAO1)
Product Performance:
Related Links:
Protein Synthesis:
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