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Description:

Human [Leu⁶⁰]Insulin-like Growth Factor-I ([Leu⁶⁰]IGF-I) is an analog of human IGF-I with a Leu substitution in place of Tyr at position 60. Human [Leu⁶⁰]IGF-I has greatly reduced binding to the Type 1 IGF receptor and to the insulin receptor compared to IGF-I, and a lower affinity than human [Leu²⁴]IGF-I. However binding to acid-stripped human serum binding proteins is unaltered. Consequently human [Leu⁶⁰]IGF-I is an extremely useful analog of IGF-I for investigating IGF actions largely independent of binding to the Type 1 IGF receptor.

References:

Bayne M.L. et al. (1990) J. Biol. Chem. 265, 15648-15652

Source:

Produced recombinantly

Purity:

> 95 % (by HPLC and N-terminal sequence analysis)

Molecular Weight:

7598.7 daltons – confirmed by Mass Spectrometry

N-terminal sequence analysis:

5 residues > 95 % single sequence

Biological Activity:

Type 1 IGF receptor binding assay: ED₅₀ > 100 ng/ml
IGF binding protein assay: ED₅₀ < 10 ng/ml
Stimulation of protein synthesis in rat L6 myoblasts: ED₅₀ > 2000ng/ml

Endotoxin:

< 0.1 EU/ µg

State and Appearance:

Lyophilized white powder
Dried from 0.1 M acetic acid and stored under dry nitrogen at a slight vacuum (-25 kPa)

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized)

Reconstitution:

#1000: Handling of GroPep Bioreagents IGF-I, IGF-II and IGF Analogs