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Description:

Human [Leu²⁴][Ala³¹]Insulin-like Growth Factor-I (IGF-I) is an analog of human IGF-I with a Leu substitution in place of Tyr at position 24 and an Ala for Tyr at position 31 of the human IGF-I sequence. Human [Leu²⁴][Ala³¹]IGF-I has greatly reduced binding to the Type 1 IGF receptor and to the insulin receptor compared to IGF-I, [Leu²⁴]IGF-I and [Leu⁶⁰]IGF-I. Human [Leu²⁴][Ala³¹]IGF-I has normal binding to acid-stripped human serum binding proteins. Consequently human [Leu²⁴][Ala³¹]IGF-I is an extremely useful analog of IGF-I for investigating IGF actions largely independent of binding to the Type 1 IGF receptor.

References:

Forbes, B.E. et al. (2002) Eur. J. Biochem., 269, 961-968
Milner, S. J. et al. (1995) Biochem. J., 308, 865-871

Source:

Produced recombinantly

Purity:

> 95 % (by HPLC and N-terminal sequence analysis)

Molecular Weight:

7507 daltons – confirmed by Mass Spectrometry

N-terminal sequence analysis:

5 residues > 95 % single sequence

Biological Activity:

Type 1 IGF receptor binding assay – ED₅₀ > 50 ng/ml
IGF binding protein assay – ED₅₀ < 10 ng/ml
Stimulation of protein synthesis in rat L6 myoblasts: ED₅₀ >2000 ng/ml

Endotoxin:

< 0.1 EU/µg

State and Appearance:

Lyophilized white powder
Dried from 0.1 M acetic acid and stored under nitrogen at a slight vacuum

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized)

Reconstitution:

#1000: Handling of Novozymes GroPep IGF-I, IGF-II and IGF Analogs