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References:

Xian C.J. et al. (1996) J. Mol. Endocrinol. 16, 89-97

Source:

Rabbit antiserum

Immunogen:

Recombinant human EGF (conjugated to ovalbumin)

State and Appearance:

Lyophilized powder

Specificity:

As determined by radioimmunoassay:
100 % cross-reactivity with Porcine EGF
56 % LONG^TMhuman EGF
< 0.01 % Mouse EGF
< 0.01 % Human TGF-α
< 0.01 % LONG^TMR³IGF-I

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized)
After reconstitution store at -20°C
Avoid freeze-thaw cycles

Reconstitution:

Dissolve in 50 µl sterile water (a 1/10 dilution).
Dilute in a buffer suitable for the desired application

Application and Titre:

Radioimmunoassay 1/500,000
At a dilution of 1/500,000 the antiserum binds 30% of fresh [¹²⁵I]hEGF tracer.
Half-maximal inhibition (ED₅₀) of radioligand binding is produced by 1.4 ng/ml of human EGF.
Limit of detection is 0.3 ng/ml.
Immunohistochemistry 1/100 - 1/600
Western Blotting 1/1000 - 1/5000

Protocols:

#3501: Determination of EGF / Betacellulin by Radioimmunoassay (RIA)

#3510: Immunohistochemical (IHC) Detection of human EGF in Paraffin Sections by Immunoperoxidase Staining

#3520: Procedure for Western Immunoblotting of EGF / Betacellulin

Related Products:

Human LONG EGF

Porcine EGF pre-RIA Pack

Porcine EGF Pack