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Description:

Human [Ala³¹]Insulin-like Growth Factor-I ([Ala³¹]IGF-I) is a 70 amino acid analog of human IGF-I comprising the complete human IGF-I sequence with the substitution of an Ala for Tyr at position 31 (hence [Ala³¹]). GroPep scientists have engineered this analog with the express purpose of changing biological activity. Human [Ala³¹]IGF-I has normal binding affinity for acid-stripped human serum binding proteins. However compared to human IGF-I, human [Ala³¹]IGF-I has reduced binding to the Type 1 IGF receptor and to the insulin receptor. Human [Ala³¹]IGF-I binds to these receptors slightly more strongly than human [Leu²⁴]IGF-I or human [Leu⁶⁰]IGF-I.

References:

Forbes, B.E. et al. (2002) Eur. J. Biochem., 269, 961-968
Milner, S. J. et al. (1995) Biochem. J., 308, 865-871

Source:

Produced recombinantly

Purity:

> 95 % (by HPLC and N-terminal sequence analysis)

Molecular Weight:

7557daltons – confirmed by Mass Spectrometry

N-terminal sequence analysis:

5 residues > 95 % single sequence

Biological Activity:

Type 1 IGF receptor binding assay: ED₅₀ > 10 ng/ml
IGF binding protein assay: ED₅₀ < 10 ng/ml
Stimulation of protein synthesis in rat L6 myoblasts: ED₅₀ < 50 ng/ml

Endotoxin:

< 0.1 EU/µg

State and Appearance:

Lyophilized white powder
Dried from 0.1 M acetic acid and stored under dry nitrogen at a slight vacuum (-25 kPa)

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized)

Reconstitution:

#1000: Handling of Novozymes GroPep IGF-I, IGF-II and IGF Analogs