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Description:

Rat Insulin-like Growth Factor-I (Rat IGF-I) is a polypeptide comprising 70 amino acids, three of which differ from the amino acid sequence of human IGF-I. Rat IGF-I has been shown to stimulate growth in rat L6 myoblasts and other cell lines. GroPep’s Receptor Grade rat IGF-I is a high quality research reagent particularly for use in studies on cellular and animal growth, for use in specific assays for rat IGF-I and other general research purposes.

References:

Shimatzu A. & Rotwein P. (1987) J. Biol. Chem. 262, 7894-7900
Upton Z. et al. (1996) J. Endocrinol. 149, 379-387

Source:

Produced recombinantly in E.coli

Purity:

> 95 % (by HPLC and N-terminal sequence analysis)

Molecular Weight:

7687 daltons – confirmed by Mass Spectrometry

N-terminal sequence analysis:

5 residues > 95 % single sequence

Biological Activity:

Type 1 IGF receptor binding assay: ED₅₀ < 10 ng/ml Stimulation of protein synthesis in rat L6 myoblasts: ED₅₀ < 30 ng/ml

Endotoxin:

< 0.1 EU/µg

State and Appearance:

Lyophilized white powder Dried from 0.1 M acetic acid and stored under dry nitrogen at a slight vacuum (-25 kPa)

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized)

Reconstitution:

#1000: Handling of GroPep Bioreagents IGF-I, IGF-II and IGF Analogs

Detection:

#3001: Procedure for the Iodination of IGFs

#3002: Determination of IGF-I or IGF-II in a Range of Species by Radioimmunoassay (RIA)

#3003: Procedure for Western Ligand Blotting using iodinated IGF-I or IGF-II

#3006: Procedure for Western Immunoblotting rat IGF-I

#3050: Immunohistochemical (IHC) Detection of human or rat IGF-I in OCT sections by Extravidin - Cy3 staining

#3051: Immunohistochemical (IHC) Detection of IGF-I in paraffin embedded sections of rat tissues by Immunoperoxidase staining

Related Products:

Rat IGF-I Pack

Any combination of 3 different IGF-Is and/or IGF-I analogs (all Receptor Grade)

IGF-I, antibody for Immunohistochemistry

IGF-I, anti-rat, (for RIA)

IGF-I Immunohistochemistry Pack

IGF-I, anti-rat, monoclonal

IGF-I, anti-rat (for Immunoblotting)

Rat IGF-I pre-RIA Pack

Product Performance:
      Protein Synthesis:




      Radioreceptor Assay: