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Description:

Biotinylated human insulin-like Growth Factor-I (biotinyl-IGF-I) is made by reacting human IGF-I with Sulfo-NHS-Biotin reagent to label the primary amines of the IGF-I molecule. A mixture of biotinylated forms are produced, the major species being di-biotinyl-IGF-I. Biotinyl IGF-I has been prepared with a spacer to minimize steric hindrance during subsequent avidin binding.

References:

Grulich-Henn, J. et al. (1998) Horm. Res. 49, 1-7

Purity:

High purity human IGF was used as the source material.

Molecular Weight:

Di-biotinyl IGF-I was confirmed as the major species by Mass Spectrometry, although mono- and tri-biotinyl species were observed.

Biological Activity:

Western ligand blot to bovine IGFBP-2

Endotoxin:

< 0.1 EU/µg

State and Appearance:

Lyophilized white powder
Dried from acetonitrile/ 0.1% trifluoroacetic acid and stored under nitrogen at a slight vacuum

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized)

Reconstitution:

#1000: Handling of GroPep Bioreagents IGF-I, IGF-II and IGF Analogs

Protocol:

#3004: Procedure for Western Ligand Blotting using biotinylated IGF-I or IGF-II

Related Products:

Human IGF-I(Receptor Grade)

Human IGF-I (Media Grade)

Mono-biotinyl human IGF-II