Biotechnology Reagents (IGFs) >> Product Support >> Protocols >> #3121 Procedure for Enzyme Immunoassay (EIA) of Human IGFBP-3

For a detailed procedure on how to perform an EIA see Protocol #3020 General Procedure for Microplate Enzyme Immunoassay (EIA)

For human IGFBP-3 EIA, use Novozymes GroPep's monoclonal antibody MAK1 (200 µg).

Reconstitute the vial in 200 µl sterile distilled or Milli-Q water. Allow to stand at room temperature for at least 10 minutes before use.

After reconstitution, a dilution of 1/8,000 will give an absorbance of 1.0 at 490 nm when reacted in an EIA plate coated with 25 ng / well of human IGFBP-3, a 1/2,000 dilution of HRP-labelled anti-mouse IgG (DAKO PO447) and a 1 mg/ml OPD substrate solution.

ED₅₀ for this assay is ~2.5 ng human IGFBP-3 per well.


© Novozymes GroPep Limited
28 Dalgleish Street
Thebarton SA 5031
PO Box 10065 BC
Adelaide SA AUSTRALIA
Telephone +61 (0)8 8354 7700
Facsimile +61 (0)8 8354 7788
Email: AUAD-bioreagents@novozymes.com
Website: www.gropep.com.au