Biotechnology Reagents (IGFs) >> Product Support >> Application Notes >> #4001: Fish IGF-I RIA Kit

Summary:

The Kit is used for measuring the circulating levels of Insulin-like Growth Factor-I (IGF-I) in the serum or plasma of a number of important aquaculture species - see list below.

Background:

Insulin-like growth factor I (IGF-I) is a naturally produced molecule of approximately 70 amino acids that stimulates growth and differentiation in many cell types. Measuring IGF-I is useful for investigating and monitoring a number of biological processes including the actions of growth hormone, many of which are mediated by IGF-I.

If measurements of IGF-I are to have any practical application, it is essential that an appropriate assay system is used. Novozymes GroPep has therefore developed a kit specifically for the finfish Aquaculture industry and researchers working in the field.

Suitable for the assay of IGF-I in the following fish species:

Atlantic salmon (Salmo salar)
Barramundi (Lates calcarifer)
Carp (Cyprinus carpio)
Coho salmon (Oncoryhnchus kisutch)
Goldfish (Carassius auratus)
Rainbow trout (Oncoryhnchus mykiss)
Red Sea bream (Snapper) (Pagrus auratus)
Southern bluefin tuna (Thunnus maccoyii)
Tilapia (Oreochromis mossambicus)

Novozymes GroPep expects that the kit will also be suitable for measuring serum IGF-I levels in fish species other than those listed. However it will be necessary for the user to check that the kit gives accurate and meaningful results by checking for parallelism with the fish IGF-I standards. Please note that quantitative equivalence should not be assumed for non-listed species.

Assay Specificity and Performance:

Sensitive:
Detects down to 0.15 ng/ml

Highly specific for fish IGF-I:
< 0.5 % cross-reactivity with human IGF-I
< 1 % cross-reactivity with human IGF-II
< 0.01 % cross-reactivity with salmon insulin

Accurate and Reproducible:
Variation within the same assay: 3 %
Variation between separate assays: 16% (n=9)

* NOT FOR USE IN HUMANS


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