Biotechnology Reagents (IGFs) >> Product Support >> Application Notes >> #1600: Handling of Novozymes GroPep NGF

General Precautions:

* Avoid freeze-thaw cycles

Stability / Storage: NGF(2.5S) in the lyophilized state can be stored at -80°C, -20°C or 2-4°C and is stable for at least 2 years lyophilized.

Handling Procedure: Novozymes GroPep Nerve Growth Factor is supplied as a lyophilized preparation in glass vials in an atmosphere of nitrogen at a slight vacuum (-25kPa). To avoid losses care should be taken to equilibrate this vacuum when opening the vial and reconstituting the peptide. An air-filled syringe may be introduced through the bung to equalize the pressure before opening.

Reconstitution and Stock Solutions: The protein should be reconstituted in sterile distilled or Milli-Q water, preferably at a concentration of 1 mg/ml. In this form it is stable for at least 3 months at -20°C or -80°C. Avoid freeze-thaw cycles.

IMPORTANT: Irrespective of size, always reconstitute the entire vial in situ and then aliquot. Each vial contains the stated amount of protein indicated on the label and up to 20 % more by mass due to the residual moisture and counter ions retained during the freeze drying process. Therefore DO NOT estimate protein content by weight or gravimetric means as this will underestimate the actual protein content by up to 20 %.


Sterilization: Novozymes GroPep products are prepared using aseptic techniques and should therefore be adequate for most purposes.

* NOT FOR USE IN HUMANS


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